CFU-EC and ECFC colonies generated from discarded cells from methods A and B. (A) Representative photomicrograph phase-contrast of an ECFC colony, which arose when the adherent cells discarded from method A were cultured in EGM-2 medium (method B). Similar colonies were observed from 4 other adult peripheral and 5 cord blood donors. Arrows indicate colony boundary and scale bar represents 500 μm. (B) Representative phase-contrast photomicrograph of a CFU-EC colony, which arose when the nonadherent cells discarded from method B were cultured by method A. Similar colonies were observed from 4 other adult peripheral and 5 cord blood donors. Scale bar represents 500 μm.