Genotype and expression of human CCL3L1. (A) The top plot shows the standard curves with the slope and the square of the Pearson correlation coefficient (R²). The bottom plot shows the amplification curves obtained using 6 (1:2) serial dilutions in triplicate, ranging from 25 to 1.56 ng human genomic DNA extracted from the cell line A431 that contains 2 copies of CCL3L1 per diploid genome. The plots were obtained using Chromo4 Continuous Fluorescence Detector System. (B) Distribution of median CCL3L1 copy numbers in HIV-1 LTNPs, HTLV-2/HIV-1 LTNPs, and HTLV-2/HIV-1^MEU subjects; P values indicate significance by the Wilcoxon test. (C) Differential CCL3L1 gene expression levels (expressed as mean of fold changes) in cellular extracts of PHA-activated PBMCs of control seronegative persons and of unstimulated PBMCs from infected persons.