Galectin-1 expression correlates with the expression levels of the CD25 marker. (A) Freshly isolated human CD4⁺ T cells were labeled with anti-CD4 and anti-CD25 mAbs and sorted according to the gates illustrated to isolate CD4⁺CD25⁻, CD4⁺CD25^dim, and CD4⁺CD25⁺ populations. (B) Histogram plot showing the expression of CD25 in the sorted populations. (C) Lysates of freshly isolated CD4⁺CD25⁻, CD4⁺CD25^dim, and CD4⁺CD25^high T cells were examined by Western blot analysis for galectin-1 expression.