Figure 1
ICSBP expression in BMMCs. (A) mRNA level of ICSBP determined by real-time PCR. The mRNA level of ICSBP was represented as the ratio to that of BMMCs without IFN-γ stimulation. Data represent mean ± SD of triplicate samples. (B) Western blotting analysis of ICSBP. BMMCs were incubated for 16 hours in the presence or absence of 100 U/mL IFN-γ. RAW264.7 cells, a mouse macrophage cell line, were used as the positive control. Total RNA (1 μg) was used for mRNA analysis and lysates of cells (2.5 × 106) were used for Western blotting. These results are representative of 3 independent experiments that had similar results.
