Transcription of IL-12–related molecules in LPS-stimulated BMMCs. RT-PCR analysis of p40, p35, and p19 after LPS stimulation for 0 to 48 hours. BMMCs were stimulated with 1 μg/mL LPS after the cells were preincubated with 100 U/mL IFN-γ for 16 hours. Equality of the RT reaction of isolated RNA was confirmed by amplification of the housekeeping gene GAPDH. These results are representative of 3 independent experiments that had similar results.