IL-12 secretion from BMMCs upon LPS stimulation. ELISA analysis of (A) p40 and (B) p70 in the culture media of cells (2 × 10⁵ cells/150 μL/well) that were incubated with the indicated concentration of LPS for 24 hours after preincubation in the presence or absence of 100 U/mL IFN-γ for 16 hours. To activate BMMCs via FcϵRI, BMMCs were incubated in the presence or absence of 50 ng/mL TNP-BSA for 4 hours (left) or indicated time from 0 to 24 hours (right) after sensitization with 1 μg/mL anti-TNP IgE for 1 hour (A). Murine PEC-MΦs were used as the positive control. (C) Comparison of p40 production level between purified BMMCs by sorting of c-Kit^high/FcϵRIα^high and nonsorted BMMCs. Both purified and whole BMMCs (1 × 10⁵ cells/150 μL/well) were incubated with the indicated concentration of LPS for 24 hours after preincubation in the presence of 100 U/mL IFN-γ for 16 hours. Data represent mean ± SD of 3 independent experiments. nd indicates not detected (detection limit is 4 pg/mL).