In vivo DMS footprinting of the Lyl1 promoter proximal region reveals extensive protections and enhancements of DMS reactivity on both DNA strands in hematopoietic progenitor (416B) but not T-cell (BW) cell lines. (A) In vivo DMS footprinting of Lyl1-P1 core promoter (sense strand). Protections (○) and enhancements (•) were observed over both Ets binding sites (EBS1 and EBS2) as well as the conserved GGCC motif in 416B compared with naked DNA (G reaction; G) and the nonexpressing T-cell line BW. (B) In vivo DMS footprinting of Lyl1-P2 core promoter (antisense strand). Protections (○) and/or enhancements (•) were observed over the 3 conserved Ets binding sites (EBS3, EBS4, and EBS5) as well as 1 of the conserved GATA sites (GBS1) in 416B compared with naked DNA (G reaction; G) and the nonexpressing T-cell line BW. (C) In vivo DMS footprinting of Lyl1-P2 core promoter (sense strand). Protections (○) and/or enhancements (•) indicate the positions of conserved Ets binding sites EBS4 and EBS5. (D) The DNA sequence of the mouse Lyl1 promoter proximal region with protections and enhancements is indicated as white and black circles, respectively. The numbers indicate the nucleotide positions relative to the alignment shown in Figure 2B.