Increased frequencies of NK and γδ T cells in full-length Helios reconstituted animals. (A) Frequency of γδ T cells among the total GFP⁺ population in spleen (SP), mesenteric lymph node (LN), and Peyer patches (PP). Data represent a total of 6 mice for each construct. Asterisks indicate a statistically significant difference between GFP control and Helios mice (spleen and mesenteric lymph node, P < .001; Peyer patches, P < .01). (B) NK-1.1 and CD3 expression in GFP⁺ spleen cells were analyzed in mice reconstituted with cells expressing the indicated retroviral vectors at 12 to 16 weeks after transplantation. (C) Frequency of NK cells among total GFP⁺ splenocytes (GFP, n = 13; Helios, n = 13; Helios Δ49-285, n = 6). Asterisk indicates a statistically significant difference between GFP control and Helios mice (P < .01). Error bars represent 1 D from the mean.