SDS-PAGE of purified fibrinogen. (A) A 4% nonreducing SDS-PAGE. The arrows indicate the migration positions of the major (340 kDa) and minor (305 kDa) circulating forms of fibrinogen, that have, respectively, fully intact chains or one proteolytically cleaved Aα-chain.²²  (B) A 7.5% reducing SDS-PAGE. (A-B) Lane 1: unrelated healthy individual; lane 2: case 1, 29 days before transplantation; lane 3: case 1, 46 days after transplantation; lane 4: case 2, immediately before transplantation; lane 5: case 2, 92 days after transplantation. Gels were stained with Brilliant blue R250. Fibrinogen preparations from cases 3 to 5 migrated in an identical manner to those shown here.