Representative ESIMS tryptic maps of purified Aα chains recorded in the m/z range 1060-1150. (A) Unrelated healthy individual. (B-D) Case 1, 29 days before (B), 46 days after (C), and 137 days after (D) transplantation. (E- F) Case 2, immediately before (E) and 92 days after (F) transplantation. The normal and variant forms of the [M+2H] ion of peptide T-62, that matches a fragment with a sequence that includes amino acid residue 526, are indicated at their predicted positions of 1133 and 1118 m/z. Before liver transplantation, equal amounts of wild-type Glu526 and variant Val526 Aα-chains were detected in the plasma fibrinogen of the fibrinogen amyloidosis patients. After liver transplantation only wild-type Glu526 chains were detected.