Loss of endogenous Bcl-2 does not reduce the severity of Eμ-myc lymphoma. (A) Comparison at time of death of spleen weights (g) from moribund Eμ-myc (n = 8), Eμ-myc/bcl-2^−/− (n = 10), and normal control (wt) reconstituted mice (n = 4), and of peripheral leukocyte cellularity (× 10⁶ cells/mL) from moribund Eμ-myc (n = 8), Eμ-myc/bcl-2^−/− (n = 9), and normal control (wt) (n = 9) reconstituted mice. Bars indicate the mean values. (B) Hematoxylin/eosin staining of representative LN (×40), bone marrow (×20), and liver (×20) sections taken from terminally ill Eμ-myc and Eμ-myc/bcl-2^−/− reconstituted animals. Images were visualized using an Olympus BX50 microscope (Olympus, Tokyo, Japan) equipped with a 40×/0.85 NA or a 20×/0.5 NA objective. Images were photographed with an Olympus U-HAD3 digital camera and recorded using Axiovision 4.2 software (Carl Zeiss, Thornwood, NY). Images were processed with Adobe Photoshop CS2 software, version 9.0 (Adobe Systems, San Jose, CA).