Survival analysis for erythroleukemic, Vegfhi/+ mice. (A) Blood was sampled weekly (4-week period) by tail vein bleeding of 8-week-old Vegfhi/+ and wt mice. VEGF-A levels were detected by using the VEGF-A ELISA kit. (B) A Kaplan-Meier survival curve was plotted for Vegfhi/+ (n = 18) and wt (n = 16) mice inoculated by F-MuLV. Mice were monitored on a daily basis and killed according to institutional guidelines. The experiment was terminated on day 56 after viral inoculation. (C) Spleens were harvested and measured for volume as an indicator of tumor burden. (D) Splenocytes derived from 8-week-old healthy and F-MuL–induced erythroleukemic wt (CD-1) and Vegfhi/+ mice (n ≥ 3/group, only 2 samples of splenocytes derived from erythroleukemic wt mice are presented in the panel) were tested for VEGF-A RNA levels. **P < .05.
