Analysis of the number and size of CFU-Es in Vegf^hi/+ mice. Eight- to 10-week-old Vegf^hi/+ and wt mice (n > 3/group) were killed, and femurs were harvested and flushed. Bone marrow cells were cultured in methylcellulose for the evaluation of CFU-Es (A) (counted after 2 days) and BFU-Es (C) (counted after 8 days). Cells were cultured with no supplement (□), 4 ng/mL rmVEGF-A₁₆₄ (⊡) or 20 μg/mL of DC101 (▪). CFU-Es derived from nonsupplemented culture (control) were imaged as described in “Methylcellulose assay for CFU-E and BFU-E measurement” for Vegf^hi/+ (B) and wt mice (D). **P < .05.