Knockdown of CD164 modulates CD133⁺ and Jurkat cell migration to CXCL12. (A) CD164 knockdown using RNAi in CD133⁺ cells. (Top) Western blot of CD164 protein levels in CD133⁺ cells that were untreated (nucleofected without siRNA) or nucleofected with control or CD164 siRNA duplexes, using α-tubulin as a loading control. (Bottom) Densitometry analysis of CD164 protein levels in RNAi-treated CD133⁺ cells (*P < .05; n = 2). (B) Transwell migration assay of CD133⁺ cells that were untreated or nucleofected with control or CD164 siRNA duplexes and induced to migrate to CXCL12 on fibronectin (**P < .001). (C) CD164 knockdown using RNAi in Jurkat cells. (Top) Western blot of CD164 protein in Jurkat cells that were untreated or nucleofected with control-2 or CD164-2 siRNA duplexes, using α-tubulin as a loading control. (Bottom) Densitometry analysis of CD164 protein levels in RNAi-treated Jurkat cells (*P < .05; n = 2). (D) Transwell migration assay of Jurkat cells that were untreated or nucleofected with control-2 or CD164-2 siRNA duplexes and induced to migrate to CXCL12 (**P < .001).