R-CM mediates BCR/ABL-independent activation of STAT-5. Western blotting. 14R-CM and 25R-CM causes a BCR/ABL-independent activation of STAT-5 in the presence of IM or NI. Activated (phosphorylated) BCR/ABL kinase (p-BCR/ABL) was demonstrated by staining for p-BCR/ABL and the phosphorylated direct substrate of BCR/ABL, p-CrkL. Activation of the BCR/ABL-substrate, STAT-5, was shown using phospho-STAT-5 (p-STAT-5)–specific antibodies. Actin served as loading control.