BJAB B cells transfected with ZAP-70 show stronger and prolonged activation of Syk, ERK, and Akt after BCR stimulation. Parental and ZAP-70–transfected BJAB B cells were stimulated with anti-IgM for the indicated times and analyzed by immunoblotting with phospho-ZAP-70^Tyr319/Syk^Tyr352, phospho-PLCγ2^Tyr1217, IκB-α, phospho-JNK^{Thr183/Tyr185}, phospho-ERK1/2^{Thr202/Tyr204}, and phospho-AKT^Ser493 antibodies. To ensure equal loading, membranes were stripped and reprobed with anti-Syk (top) or anti-ERK1/2 and anti–β-actin (bottom).