aPC suppressed hypotension and prolonged survival in low-PC mice. The mean arterial blood pressures (MAPs) in LPS-challenged WT (A) and low-PC mice (B). The onset of LPS injection (2 μg/g) is indicated by the arrow (time 0). Continuous infusion of rhaPC to PC^−/−(PC^Tg785) mice resulted in a steady-state aPC concentration of 100 ng/mL at 15 hours. By 24 hours, the rhaPC concentration was 60 ng/mL (C). The data represent means of 5 mice per group ± SEM. Survival analysis of PC^−/−(PC^Tg785) mice challenged with LPS and treated with rhaPC (solid line) or saline (dotted line) (D). The data represent 16 to 25 mice/treatment group. Mice were challenged with LPS and infused with rhaPC or saline for 24 hours. Mice treated with rhaPC showed improved MAPs and overall longer survival (F) compared to saline-treated mice (E). In all graphs, solid lines represent mice that survived and dashed lines represent nonsurvivors.