Expression of NKp30 or NKp46 ligands on HIV-infected primary T cells. (A) The COS-7 cell line was stained with either soluble NKp30-Fc or NKp46-Fc fusion proteins followed with goat antihuman secondary reagent or the secondary reagent alone. (B) Uninfected and (C) HIV-1_SF162–infected CD4⁺ T cells were stained with soluble NCR as in panel A, fixed and permeabilized, and stained with mouse anti-HIV-1 p24 monoclonal antibodies and rabbit antimouse secondary reagent. Histograms were gated on 10⁴ viable (panels A,B) or 10⁴ HIV-1 p24 Ag⁺ cells (panel C). Dot plots shown are of 3 × 10⁴ cells in the viable gate from cultures of T-cell blasts infected with HIV-1_SF162 (panels D,E) and HIV-1_SF128A (panels F,G), and the numbers represent the percentage of viable cells within each quadrant. Data are representative of 3 separate studies.