Expression of NKp44 ligands on HIV-infected primary T cells. (A,C) uninfected and (B,D) HIV-1_SF162– or HIV-1_SF128A–infected CD4⁺ T cells stained with soluble NKp44/IgG Fc fusion protein and goat antihuman secondary reagent followed by intracellular staining with mouse anti-HIV-1 p24 antibodies. Dot plots shown are of 3 × 10⁴ cells in the viable gate from cultures of T-cell blasts infected with HIV, and the numbers represent the percentage of viable cells within each quadrant. (E) The percentage of HIV-1 p24 Ag⁺ (–) and p24 Ag⁻ (———) infected cells that are NKp44 ligand-positive. Data are representative of 3 separate studies.