Maturation of mDC subsets after cytokine induction. (A) Expression of CD40 and CD80 molecules after the induction with the indicated endogenous stimuli for the reported time period in CD16 (left panel) and CD1c (right panel) mDCs. Fluorescence histograms with percentage of positive cells for both markers are reported. Representative data of one donor out of 3. (B) IL-6 secretion by mDC subsets after 40-hour stimulation with TNF-α, IL-3, type I interferons. (C) IL-6 secretion by mDC subsets in the same conditions as B on Pam stimulation. Note the difference in scale between B and C. Representative data of one donor out of 3.