Figure 6
![Figure 6. Structure of VB22B sc(Fv)2 minibody. A noncovalent (55 kDa) “diabody” (middle of figure) can be generated by taking the VH and VL regions of a whole anti-Mpl monoclonal IgG (left) and coupling them via a 5-amino acid linker (GGGGS). VB22B sc(Fv)2 is made by binding one VH and one VL fragment together with a 15-amino acid linker [(GGGGS)3] and then binding 2 of these constructs together with the same 15-amino acid linker (middle). Both the diabody and the VB22Bsc(Fv)2 bind and activate the TPO receptor (right). Illustration by Paulette Dennis.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/109/11/10.1182_blood-2006-10-019315/4/zh80110703760006.jpeg?Expires=1735504764&Signature=A0n3AiBgHzZfaniW4BrXrSQUM1il3V7PPmt-Tu2qWqzjz8jiNsrwbH-M2TxwSYcQxq8FmywV7bl~SgTn07J7lrofqOXs1GyxIu88n6yMmH2vye285vukICwlovECxBKiMjeFBc2XDvHXs2xgLneXxGpQxI-iZWIwumHb6DYYuyfnUYQzAOWBUAujezGX95UxjBMCRyimBFztC3LIYsYU42y1SeurpEVAZoJltdSw4g9PNGHJwDBWx7fIN66nQagheG7jewB-E29YBoGKhAq1w68X6oMwRwaoTd~IfvfBF3gubVel~BnH1tQ6e6IKvcuLzH6vFJHa0rsKqc8dnrWCxg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Structure of VB22B sc(Fv)2 minibody. A noncovalent (55 kDa) “diabody” (middle of figure) can be generated by taking the VH and VL regions of a whole anti-Mpl monoclonal IgG (left) and coupling them via a 5-amino acid linker (GGGGS). VB22B sc(Fv)2 is made by binding one VH and one VL fragment together with a 15-amino acid linker [(GGGGS)3] and then binding 2 of these constructs together with the same 15-amino acid linker (middle). Both the diabody and the VB22Bsc(Fv)2 bind and activate the TPO receptor (right). Illustration by Paulette Dennis.
