Functional analysis of miR-16. The NZB malignant B-cell line, LNC, was transfected with either the miR-16 or control mimics. The cells were harvested 24 hours after transfection, and the cell-cycle stages were determined by flow cytometric techniques. The columns represent the mean percentage change in the NZB B-cell line treated with miR16 versus control mimic treatment (n = 4). Error bars indicate mean ± SEM.