Definitive HSPCs in the PBI can populate pronephros. (A, B) Lateral view of 2-dpf (A) and 3-dpf (B) fish indicates the uncaging positions (cross 1 and 2). (C, D) Confocal images of the flu and c-myb signals in the 5-dpf kidney. The embryos were uncaged at cross 1 in panel A, fixed at 5 dpf, and co-stained with flu and c-myb RNA. (C/D) Merged image of panles C and D. (C'/D') Superimposed view of panels C/D and DIC image. (E, F) Confocal images of the flu and c-myb signals in the 5-dpf kidney. The embryos were uncaged at cross 2 in panel A, fixed at 5 dpf, and co-stained with flu and c-myb RNA. (E/F) Merged image of panels E and F. (E'/F') Superimposed view of panels E/F and DIC image. Arrows indicate the co-staining of flu and c-myb.