Figure 2
![Figure 2. Minigene analyses of ankyrin mRNA splicing. Wild-type and mutant ankyrinAnkara minigenes from exon 15 to exon 20 were prepared and transfected into COS and K562 cells. The sequence of the RT-PCR products derived from RNA of transfected cells was analyzed. RNA from cells transfected with the wild-type minigene contained the expected normally sized and spliced product (wild-type [WT], top line). RT-PCR products derived from RNA of cells transfected with the mutant ankyrinAnkara minigene yielded 11 mutant spliceoforms, shown schematically, but no wild-type product. Similar studies with reticulocyte RNA from the proband yielded the same results, except that the +26-bp and +144-bp spliceoforms were not found.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/109/12/10.1182_blood-2006-09-046573/4/zh80120701240002.jpeg?Expires=1735570300&Signature=srrof-NmqW9lSA7v60OlDqPYe7scTzIV-V6pKI8GVKgHuoORGdLLnGy10olWbpKCarZ9jY~iuHgbUStD2fAKv6ap1114-du3hievQEpq~wi4ueje-4ozfrgmMjchXE-HffMcZ8JgA3tAiEZz-GiwwRnMysjW2RM-5purgsQQumDBdBvMIodEeVBs4p8XUTEdiLG9ierW~KP03ZeKNIp-qoosEyMDI3XpO6DFOXPZOLIOcNrIc-LnPH~Whhn-Gy3ngbO7lYHwqw-7XAnDa1Vs7W6bgWr2Cl329XLTWxZFlBQ5hnFcWXwfpGSJFqlhx8vP~F0zDwDNVG64EuGGnG0Krw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Minigene analyses of ankyrin mRNA splicing. Wild-type and mutant ankyrinAnkara minigenes from exon 15 to exon 20 were prepared and transfected into COS and K562 cells. The sequence of the RT-PCR products derived from RNA of transfected cells was analyzed. RNA from cells transfected with the wild-type minigene contained the expected normally sized and spliced product (wild-type [WT], top line). RT-PCR products derived from RNA of cells transfected with the mutant ankyrinAnkara minigene yielded 11 mutant spliceoforms, shown schematically, but no wild-type product. Similar studies with reticulocyte RNA from the proband yielded the same results, except that the +26-bp and +144-bp spliceoforms were not found.
