More efficient missing self-reactivity in mice expressing multiple MHC class I alleles. (A) Remaining CFSE⁺ donor spleen cells in the blood 2 days after injection. Numbers indicate the relative survival in the test population (CFSE^high) compared with the syngeneic control population (CFSE^low). Graphs show data from 1 representative mouse. (B) Summary of all 3 experiments performed. The dashed line represents complete survival, and error bars denote SD. Each data point represents n = 2-6. MHC^−/− versus D^b: *P < .05; D^b versus K^bD^b: ***P < .001; K^bD^b versus K^bD^bD^d: nonsignificant. depl. indicates depleted; untr., untreated.