PGE2 enhances T-cell-stimulatory capacities of human MoDCs. Human CSFE-labeled PBMCs were stimulated with tetanus toxoid (TT; A) or keyhole limpet hemocyanin (KLH; B) pulsed autologous immature (iDC) and MoDCs matured with trimeric soluble CD40L (mDC) in the absence or presence of PGE2. On day 6 of the coculture, cells were harvested, stained for CD3, CD4, CD8, and Sytox Blue to identify live T cells, and analyzed by flow cytometry. CSFE fluorescence of live CD3+CD4+ and CD3+CD8+ T cells from a representative experiment of at least 5 is shown. Percentages of proliferating, CSFE low, T cells are indicated. (C) Immature (iDC) or matured MoDCs (mDC) were analyzed for the expression of costimulatory molecules by flow cytometry. MoDCs were matured with sCD40L in the absence or presence of PGE2. Gray thin lines represent isotype controls; black bold lines, specific staining for CD83, CD80, or CD86 as indicated.
