Enhanced expression of OX40L, OX40, and CD70 on T cells stimulated with PGE₂-matured MoDCs. MoDCs were matured with sCD40L in the absence or presence of PGE₂, pulsed with TT (A,C,E) or KLH (B,D,F), and cocultured with autologous PBMCs. On days 2 and 6 of the coculture, surface expression of OX40L (A,B), OX40 (C,D), and CD70 (E,F) was analyzed on CD3⁺CD4⁺ or CD3⁺CD8⁺ T cells by flow cytometry. (A-D) One representative experiment on day 6 is shown as dot plots; diagrams represent mean values and SEM for at least 5 independent experiments with different donors. (E,F) Mean values and SEM of at least 4 independent experiments at day 6 are presented. *P < .05; **P < .01.