Lad associates with Gβ. Cos7 cells were transfected with the indicated combinations of expression plasmids encoding Lad, Gβ, and/or Gγ. After incubation for 48 hours, the cell lysates were subjected to immunoprecipitation with the antiFLAG antibody to precipitate FLAG-tagged Lad. This was followed by Western blotting with antiGβ (upper panel) or antiFLAG (lower panel) antibody.