Figure 3
Figure 3. In vitro studies of the effect of PF4 and blocking antibodies. (A) The effect of recombinant human or mouse PF4 on numbers of megakaryocyte colonies from WT marrow and the ability of species-specific polyclonal antibodies to block this effect. Relative means of megakaryocyte colonies are shown + 1 SD *P < .02, **P < .005, and ***P < .001 each compared with untreated WT. The star indicates P < .03 compared with WT + PF4 of same species. (B) Relative means + 1 SD of megakaryocyte colonies compared with WT controls for mPF4−/− and hPF4×6+ mice are shown. *P < .04 compared with WT, **P < .001 compared with WT. (C) The effect of adding anti-hPF4 antibodies to bone marrow from hPF4×6+ animals. *P < .009 compared with hPF4×6+ baseline. **P < .001 compared with WT. Data were normalized to WT in each experiment in all panels to control for interexperiment differences in total number of colonies obtained.

In vitro studies of the effect of PF4 and blocking antibodies. (A) The effect of recombinant human or mouse PF4 on numbers of megakaryocyte colonies from WT marrow and the ability of species-specific polyclonal antibodies to block this effect. Relative means of megakaryocyte colonies are shown + 1 SD *P < .02, **P < .005, and ***P < .001 each compared with untreated WT. The star indicates P < .03 compared with WT + PF4 of same species. (B) Relative means + 1 SD of megakaryocyte colonies compared with WT controls for mPF4−/− and hPF4×6+ mice are shown. *P < .04 compared with WT, **P < .001 compared with WT. (C) The effect of adding anti-hPF4 antibodies to bone marrow from hPF4×6+ animals. *P < .009 compared with hPF4×6+ baseline. **P < .001 compared with WT. Data were normalized to WT in each experiment in all panels to control for interexperiment differences in total number of colonies obtained.

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