LPS induces Gata1 expression in DCs in vitro and in vivo. (A) qRT-PCR analysis of gene expression in GM-CSF cultures 12 hours and 60 hours upon LPS stimulation. Data are derived from 4 to 8 independent samples obtained in 2 experiments and analyzed in triplicate. (B) qRT-PCR analysis of gene expression in CD8⁻ mDCs and pDCs sorted from the spleen of LPS-treated (+ LPS) and nontreated (+ PBS) mice. Hprt1, Gapdh, and ubiquitin were used as controls. (C top) qRT-PCR analysis of gene expression in GM-CSF cultures 60 hours upon LPS stimulation and relative to untreated samples. (Middle) qRT-PCR analysis of gene expression in GM-CSF cultures treated with OHT for 36 hours, relative to untreated samples. (Bottom) qRT-PCR analysis of gene expression in LPS-stimulated GM-CSF cultures treated with OHT for 36 hours and relative to LPS-stimulated control samples. Data are derived from 4 to 8 independent samples obtained in 2 experiments and analyzed in triplicate. The average (± SD) of the enrichment (fold increase) relative to nonstimulated samples (set to 1) is depicted. Gapdh and Hprt1 were used as controls. RFE indicates relative fold enrichment. The P values are derived from Mann-Whitney statistical analysis of independent samples. ND indicates not detectable.