Gata1 regulates Ifng in LPS-stimulated mDCs. (A) Multiple sequence alignment of the proximal Ifng promoter in human (Homo sapiens), mouse (Mus musculus), rat (Rattus norvegicus) and dog (Canis familiaris). The potential GATA binding site is highlighted. (B) Gata1 ChIP analysis of the Ifng promoter in mDCs derived from GM-CSF cultures stimulated for 3 days with LPS (+ LPS) or grown under standard conditions (ST) starting from day 8 of culture. Amplicon containing GATA binding site (GATA+); amplicon not containing a GATA binding site (GATA−). Average (± SD) of at least 2 independent experiments performed in triplicate is shown. Corresponding isotype ChIPs (rat IgG) are depicted. RFE indicates relative fold enrichment. (C) ELISA on KO and WT GM-CSF culture supernatants stimulated with LPS for 60 hours or grown under standard conditions (ST). Expression of IFN-γ and IL-6 (average ± SEM of 3 independent samples) is shown. ND indicates not detectable.