Flow cytometric analyses of CB-derived MNCs that have been cultivated for 3 days in the presence of early-acting cytokines. Plots represent MNCs that are ungated or gated on CD34⁺ cells. The CD34⁺ gate used is shown in the first plot of the first row. The size of these cells plotted against their granularity is shown in the second plot of row 1. The remaining plots represent the intensity of CD133 staining against the intensity of the isotype control, an anti-CD38, anti-CD47, anti-CD53, anti-CD62L, anti-CD63, or anti-CD71 staining, respectively. Quadrants are adjusted according to isotype controls of CD34 negative cells. Note that CD34⁺CD133⁺ cells contain different levels of CD53, CD63, CD62L, and CD71 than CD34⁺CD133^low/− cells.