Inhibitor sensitivity of currents induced by H734R mutation of hAE1. In these experiments, oocytes were bathed in MBS medium without bicarbonate and divalent anions to avoid precipitation with divalent cations. (A) Current/voltage curves of control oocytes expressing wt hAE1 in the absence of inhibitors (●, mean of 10 oocytes) or in the presence of 0.5 mM SITS (▲, mean of 5 oocytes) or 1 mM Zn²⁺ (□, mean (± SEM) of 8 oocytes). (B) Current/voltage curves of oocytes expressing H734R mutation in the absence of inhibitor (●) or in the presence of 0.5 mM SITS (▲) or 1 mM Zn²⁺ (○). Data are mean (± SEM) of 10 oocytes. (C) The graph bar summarizes the effect of some inhibitors on the conductance of H734R-expressing oocytes. The conductance was calculated from I/V curves between − 80 and − 20 mV in the absence of inhibitors and expressed as 100%. The conductance calculated in the presence of amiloride (0.1 mM), NPPB (0.5 mM), Zn²⁺ (1 mM), La³⁺ (1 mM), or SITS (0.5 mM) was expressed in percentage of the corresponding control oocytes and compared with the control by Student t test. (*P < .05).