Reversal of T-cell anergy after lymphodepletion and EBV-specific CTL infusion. (A) CD45 mAb-mediated lymphodepletion results in transient decrease of CD4⁺, CD25^bright, and CD69⁻ T cells (*P < .05). (B) The frequency of pentamer-positive and IFN-γ–secreting T cells specific for the HLA-A11–restricted EBNA3B peptide AVF were determined before lymphodepletion and 6 weeks after EBV-specific CTL infusion. Whereas the frequency of AVF-specific T cells was similar by pentamer analysis at both time points, AVF-specific T cells only secreted IFN-γ upon AVF peptide stimulation 6 weeks after CTL infusion.