Leukocyte recruitment in CXC chemokine-superfused cremasteric postcapillary venules in WT and PI3Kγ^-/- mice. (A) The flux of rolling leukocytes, (B) leukocyte adhesion, (C) leukocyte emigration in response to MIP-2, and (D) leukocyte emigration in response to KC in cremasteric postcapillary venules are shown. After measurement of basal leukocyte recruitment (at time 0), leukocyte recruitment was induced by superfusion of the cremaster muscle preparation with 5 nM MIP-2 or KC as indicated (arrow) and the recruitment parameters determined in cremasteric venules (n = 3 to 4 in each group). *P < .05 and **P < .01 as compared with each value in WT mice. Error bars represent means plus or minus SEM.