Leukocyte recruitment in chemokine MIP-2-superfused cremasteric postcapillary venules in chimeric mice. (A) The flux of rolling leukocytes, (B) leukocyte rolling velocity, (C) adherent, and (D) emigrated leukocytes in MIP-2-superfused cremasteric postcapillary venules of chimeric mice are shown. WT and PI3Kγ^-/- mice were reconstituted with PI3Kγ^-/- and WT bone marrow and indicated as PI3Kγ^-/-→WT and WT→ PI3Kγ^-/-, respectively. After measurement of basal leukocyte recruitment (at time 0), leukocyte recruitment was induced by superfusion of the cremaster muscle preparation with 5 nM MIP-2 (arrow) and the recruitment parameters determined in cremasteric venules from these chimeric mice (n = 4 in each group). *P < .05 and **P < .01 as compared with each group of opposite chimeric mice. Error bars represent means plus or minus SEM.