The effect of LY294002 on leukocyte emigration induced by 4- to 5-hour MIP-2 treatment in PI3Kγ^-/- mice. The number of emigrated neutrophils in cremasteric postcapillary venules after intrascrotal injection of MIP-2 (1 μg in 200 μL saline) in PI3Kγ^-/- mice with or without LY294002 was determined (n = 4 in each group). The mice were pretreated with intraperitoneal injection of either LY294002 (10 mg/kg body weight in 0.5 mL saline) or vehicle DMSO 20 minutes before MIP-2 intrascrotal injection. Leukocyte emigration was determined by intravital microscopy at 4 hours, 4.5 hours, and 5 hours after MIP-2 treatment. **P < .01 as compared with PI3Kγ^-/- mice treated with only vehicle. Error bars represent means plus or minus SEM.