Leukocyte recruitment in cremasteric postcapillary venules of saline-treated WT and TNFα-treated WT and PI3Kγ^-/- mice. (A) The leukocyte rolling flux, (B) rolling velocity, (C) adhesion, and (D,E) emigration are shown. Leukocyte recruitment was induced by intrascrotal injection of TNFα (0.5 μg in 200 μL saline) and the recruitment parameters determined in cremasteric venules from WT (n = 3) and PI3Kγ^-/- mice (n = 3) at 4 hours, 4.5 hours, 5 hours, and 7.5 hours. In panel E, PI3Kγ^-/- mice were pretreated with either vehicle PEG-400 or IC87114 at 1 hour prior to TNFα injection. The WT control mice (n = 4) were injected only with saline. τ, P < .05 and ττ, P < .01 as compared with saline-injected WT control group. *P < .05 and **P < .01 as compared with TNFα-treated WT mice (D) or TNFα-treated PI3Kγ^-/- mice with PEG-400 (E). Error bars represent means plus or minus SEM.