Leukocyte recruitment in cremasteric postcapillary venules of TNFα-treated chimeric mice. (A) The leukocyte rolling flux, (B) rolling velocity, (C) adhesion, and (D) emigration are shown. WT and PI3Kγ^-/- mice were reconstituted with PI3Kγ^-/- and WT bone marrow, indicated as PI3Kγ^-/-→WT and WT→ PI3Kγ^-/-, respectively. Leukocyte recruitment was induced by intrascrotal injection of TNFα (0.5 μg in 200 μL saline) and the recruitment parameters determined in cremasteric venules from these chimeric mice (n = 5 in each group) at 4 hours, 4.5 hours, and 5 hours. **P < .01 as compared with groups of opposite chimeric mice. Error bars represent means plus or minus SEM.