In vivo maturation of DCs by different TLR-Ls. C57BL/6 mice received an intravenous injection of PBS or zymosan, poly(I:C), LPS, poly(U), R848, or CpG-B. CpG was administered either naked or mixed with DOTAP. (A) The up-regulation of MHC-I (H-2K^b) and CD86 molecules was analyzed on purified splenic DCs 15 hours after treatment. (B) Intracellular staining of IL-12 was analyzed on sorted splenic DCs 4 hours after TLR injection. (A-B) Cells were gated on the CD11c^high population. Results are representative of 2 independent experiments.