Trif dependence of poly(I:C) adjuvant properties. (A) Trif^−/− mice and their Trif^+/+ littermates received a single intravenous injection of poly(I:C) (25 μg), and, various times after injection, their sera were titrated for IFN-α, IFN-β, IL-12p40, IL-6, and MIG by ELISA. Results are expressed as means ± SEM for 4 mice per group, tested in 2 independent experiments. (B) Trif^+/+ and Trif^−/− mice were left untreated or were immunized via the intravenous route with BOVAp either alone or in combination with poly(I:C). The specific CTL response was analyzed by tetramer staining on day 7. Data are representative of 2 independent experiments.