Effect of PKA-c on basal SHP-1 P2 promoter activity and TIPS. Jurkat-LT cells were transfected with different combination of plasmids. Dosages of plasmids used were as follows: wt-P2-Luc, 500 ng; wt Tax, 200 ng; RelA, 300 ng; CBP, 100 ng; p300, 100 ng; PKA-c, 400 ng. Luciferase activity was measured as previous described. (A) Effect of PKA-c on CBP, p300 activation of SHP-1 P2 promoter in the absence or presence of Tax. (B) Effect of PKA-c on RelA activation of SHP-1 P2 promoter in the presence or absence of CBP, p300, and Tax. Cell lysates were assayed in triplicates for luciferase activity and values represent the mean ± SD of 2 experiments. The relative luciferase activity was normalized against basal SHP-1 P2 promoter luciferase reporter alone (set as 100). Data presented in panels A-B were collected simultaneously, separated only for clearer illustration, and thus can be compared directly against each other.