Comparison of c-kit expression on fetal liver and adult bone marrow cell populations that are highly enriched in their CRU content. (A) Representative profile of E14.5 viable, lin⁻ fetal liver (FL) cells assessed for Sca1 and CD43 expression (left panel), further analyzed for the level of surface c-kit protein expression (right panel). (B) Representative profile of viable lin⁻Rho⁻SP adult bone marrow (BM) cells (left and middle panels) assessed for the level of surface c-kit protein expression (right panel). (C) Comparison of the geometric mean fluorescence intensity (MFI ± SEM, n = 2) of surface c-kit protein expression in the lin⁻Sca1⁺CD43⁺ fetal liver cells (shown in panel A) and lin⁻Rho⁻SP adult bone marrow cells (shown in panel B). (D) Comparison of the average fold-change (± SEM, n = 3) in c-kit gene expression relative to Gapdh between the lin⁻Sca1⁺CD43⁺ fetal liver cells (shown in panel A) and lin⁻Rho⁻SP adult bone marrow cells (shown in panel B).