Cells harvested from oc/oc+tcirg1 mice can be in vitro–differentiated to osteoclast-like cells in normal numbers. BM cells from oc/oc+tcirg1 mice were cultured for 6 days with M-CSF and RANK-L after which generation of osteoclast-like cells was analyzed. (A) Both GFP⁺ (i-ii) and GFP⁻ (iii-iv) osteoclast-like cells were formed. (i, iii) Green fluorescent; (ii, iv) blue fluorescent (DAPI) image. Bar represents 100 μm. Microscope: Leica DM IL PLAN 40× magnification, 0.5 lens; camera: Leica DFC320; and acquisition software: Leica IM500. (B) Osteoclasts were cultured on bone slices from BM cells obtained from WT or oc/oc+tcirg1 mice. Oc/oc+tcirg1 cells were also FACS sorted into GFP⁺ and GFP⁻ populations. After culture with M-CSF and RANKL for 6 days, the number of multinucleated (≥ 3 nuclei) cells was enumerated. Mean ± SD is shown.