Cytokine production of GP-iTreg. GPIIb/IIIa-reactive T cells (2 × 105) were stimulated with GPIIb/IIIa-pulsed DCs (2 × 104) in the presence or absence of GPIIb/IIIa-specific iTreg (2 × 105). Cell-free supernatants were harvested from stimulated cultures after 72-hour incubation to assess for IL-10 and active TGF-β1 concentrations by ELISA. Results represent the mean plus or minus SD for 16 independent experiments.