Inhibition of proliferation of GP-reactive T cells is independent on IL-10 or TGF-β. GPIIb/IIIa-specific iTreg (2 × 105) were cocultured with GPIIb/IIIa-pulsed DCs (2 × 104) and CFSE-labeled GPIIb/IIIa-reactive T cells (2 × 105) in the presence of neutralizing antibodies against IL-10 (10 μg/mL) and TGF-β (40 μg/mL), alone or in combination. In some experiments, IL-2 (100 μg/mL) was added to the culture system. After 3 days of coculture, the fluorescence intensity of CFSE-labeled GP-reactive T cells was determined by flow cytometry. Proliferation was measured as the percentage of proliferating GP-reactive T cells. Results are representative of 16 independent experiments (A) and shown as mean plus or minus SD with error bars representing SD (B).
