GVHD T cells can generate complex T-cell repertoires early after transplantation. Lethally irradiated (1100 cGy) AKR mice were transplanted with Rag-1 BM (5 × 106) and 5 × 105 T cells that were obtained 19 to 20 days after BMT from B6→AKR chimeras as in Figure 1. Spleen cells from individual chimeras were then each transplanted into 2 secondary AKR hosts, and animals were serially bled at days 38 and 66 days after BMT. Spectratypes from 2 mice that received splenocytes from mouse A (see Figure 3) are depicted. Repertoire skewing of input splenocytes when present is shown in lane S. Spectratypes of selected BV families from serial peripheral blood (B) samples are shown at 38 and 66 days after BMT, whereas the corresponding T-cell repertoire in the spleen (S) is depicted 90 days after transplantation. Data in these studies were obtained from mice whose day 90 splenic T-cell repertoires are also depicted in Table 1, Experiment 1. ND indicates not done because of technical failure.
