Anti-CD99L2 and anti-CD99 antibodies specifically react only with their corresponding antigen. (A) Immunoblots of CD99-Fc, CD99L2_S-Fc, and ESAM-Fc (as indicated above) with affinity-purified antibodies against CD99 and CD99L2 (as indicated below). (B) Immunofluorescence staining of COS-7 cells transiently transfected with full-length CD99L2_s (top panel) or full-length CD99 (bottom panel) using affinity-purified antibodies against CD99 or CD99L2 (as indicated on the left). Bar = 25 μm.