Frequencies of donor cell subsets in the peripheral blood and hematopoietic tissues after transplantation. New Zealand black × New Zealand white mice were conditioned with lethal irradiation and transplanted with purified allogeneic hematopoietic stem cells. (A) Donor cell frequencies of the B-cell, T-cell, and myeloid compartments of the peripheral blood at 32 weeks after transplantation are shown. The frequency of donor T cells in the total T-cell pool was lower than donor contribution to the B-cell and myeloid compartments (P < .001). Circles indicate individual mice. Horizontal lines represent mean of each group. (B) Frequencies of donor cell subsets in hematopoietic tissues of mice that received purified allogeneic hematopoietic stem cells. Tissues of recipient mice were analyzed by flow cytometry when moribund or at the conclusion of the study. Donor T-cell (green) frequency was lower than B-cell (blue) and myeloid (red) frequencies in the blood, bone marrow, lymph nodes, and spleen. Donor B-cell contribution was significantly lower in the peritoneal cavity than in the other tissues (P ≤ .001). The peritoneal cavity was analyzed for B cells only. Data were combined from 3 experiments.