Analysis of proteinuria, circulating immune complexes, and autoantibodies in New Zealand black × New Zealand white mice after nonmyeloablative conditioning and transplantation with allogeneic hematopoietic stem cells. (A) Bar graphs illustrate the frequencies of mice with proteinuria (urine protein 1 g/L [100 mg/dL] or higher) after transplantation. Seventy percent of mice that received allogeneic hematopoietic stem cells (red) did not have proteinuria after transplantation; median age at testing was approximately 506 days. In contrast, 70% of the mice receiving conditioning only (blue) and 100% of the age-matched control mice (gray) developed proteinuria before death. (B) New Zealand black × New Zealand white mice that received allogeneic hematopoietic stem cells had a reversal of their disease as measured by a decrease in the frequency of mice with proteinuria after transplantation. Bar graphs indicate the frequency of New Zealand black × New Zealand white mice with proteinuria, urine protein 1 g/L (100 mg/dL) or higher, before (blue) and after (green) treatment. The frequency of proteinuria in the mice that received transplants decreased after transplantation, whereas the frequency of proteinuria in mice that received conditioning regimen only increased. The difference in frequency of proteinuria in mice after treatment was significant (P = .015). All age-matched control mice developed proteinuria before death. (C) Frequency of mice with positive titers of circulating immune complexes or autoantibodies. The frequency of positive titers of circulating immune complexes or anti-dsDNA and antihistone autoantibodies was lower in mice that received allotransplants (red) than in mice that received conditioning only (blue) or the age-matched controls (gray).